occupation 1: Enzyme Activity
Name:
Instructor:
era:
PREDICTIONS
1. Sucrase will have the greatest exertion at:
2. Sucrase will have the greatest occupation at:
3. Sucrase activity:
MATERIALS AND METHODS
frame of pH on Enzyme Activity
1. Dependent Variable
2. autarkic Variable
3. Controlled Variables
imprint of Temperature on Enzyme Activity
1. Dependent Variable
2. self-sufficing Variable
3. Controlled Variables
Effect of Substrate Concentration on Enzyme Activity
1. Dependent Variable
2. Independent Variable
3. Controlled Variables
4. Describe what is measured as an indicator of invertase activity and why this is an indicator of saccharase activity.
5. Explain why change sucrase was used as a control.
6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase forrader measuring product concentration.
RESULTS
capture bow 1: Effect of pH on Sucrase Activity
See Graph, Effect of pH on Sucrase Activity
1. State the optimum pH for sucrase activity and take up how sucrase activity changes at more(prenominal) acidic and more alkaline pH values.
See Table 2: Effect of Temperature on Sucrase Activity
See Graph, Effect of Temperature on Sucrase Activity
2. Was the rate of increase of sucrase activity higher at a pH of 8.5 or a pH of 5.5?
3. State the optimum temperature for sucrase activity and describe how sucrase activity changes at lower and higher temperatures.
See Table 3: Effect of Sucrose Concentration on Sucrase Activity
See Graph, Effect of Sucrose Concentration on Sucrase Activity
4. Was sucrase activity higher at 25 ºC or 55 ºC?
5. State how sucrase activity changes with increasing sucrose concentration.
6. Was the rate of increase of sucrase activity greater when sucrose concentration went from 2.5 to 7.5 g/l or when it went from 22.5 to...If you want to get a full essay, parade it on our website: Orderessay
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